Scientific Research Services | Brain Case Neural Circuit Tracing Service

Release time：2024-10-15 17:10:33

Ⅰ. Why Conduct Neural Circuit Research?

1. In recent years, neurodegenerative diseases related to the nervous system have become increasingly common.
2. The incidence of neurological disorders is growing year by year, leading to a steadily increasing economic burden on society.
3. Neurological and psychiatric disorders are closely related to abnormalities in neural circuits: conducting neural circuit research helps to reveal the mechanisms of brain function; it aids in identifying potential biomarkers for diseases such as depression, schizophrenia, and autism, which can be used for early diagnosis and monitoring of these conditions; by understanding the signal transmission and coding mechanisms of neural circuits in the brain, the performance and reliability of brain-computer interfaces can be improved, enabling more natural and efficient human-computer interaction.

Ⅱ. How Can Brain Case Biotech Support Neural Circuit Research?

The Brain Case animal experimentation platform can undertake and conduct research related to neural structural circuits and neural functional circuits.

Animal Experiment Services

Animal Platform — High-Priority Research Topics on Circuits

Ⅲ. Why choose Brain Case?
We are a research team from the Chinese Academy of Sciences with extensive experience in viral development. Every critical step is strictly overseen by professional teams, including BD, SD, PM, and QA experts. We have SPF-grade animal facilities and P2 laboratories, equipped with state-of-the-art research equipment, including high-resolution microscopes, cell culture facilities, and molecular biology laboratories.
We offer highly customized services to meet the specific needs of different clients, emphasizing communication and feedback to ensure service transparency and customer satisfaction. We adhere strictly to ethical and scientific standards, ensuring the quality and compliance of our research.

Experimental Project Service Process

Ⅳ. Case Study

1. Sparse Labeling (CNS)

Virus Information: CSSP-YFP-8E3
Injection Dose: 100 nl
Animal Information: Thy1-Cre Mice
Injection Site: Primary Motor Cortex M1
Expression Period: 3 weeks
Sample Information: Frozen Sections, 50 µm Thickness

Brain Case Test Data

2. Retrograde Labeling (CNS)

Virus Information: rAAV2/Re-CaMKIIα-EGFP
Injection Dose: 200 nl
Animal Information: C57 Mice
Injection Site: BPn
Expression Period: 3 weeks
Sample Information: Frozen Sections, 50 µm Thickness

Brain Case Test Data

3. Retrograde Mono-synaptic Tracing (CNS)

Virus Information: rAAV-EF1α-DIO-EGFP-T2A-TVA, rAAV-EF1α-DIO-N2cG, rAAV-hSyn-SV40 NLS-Cre, CVS-EnvA-G-△tdTomato
Animal Information: C57 Mice
Injection Site: Primary Motor Cortex M1
Injection Dose: Helper Virus TVA: RVG: Cre = 1:2:1 (Mixed Injection 100 nl), RV injected after 14 days of expression
RV Infection Period: 7 days
Sample Information: Frozen Sections, 50 µm Thickness

Brain Case Test Data

4. Retrograde Muti-synaptic Tracing (Peripheral- Central nervous system)

Virus Information: PRV-CAG-EGFP, PRV-CAG-3MS
Injection Dose: 3 µl (each)
Animal Information: C57 Mice
Injection Site: Bilateral Leg Muscles
Expression Period: Approximately 6 days
Sample Information: Frozen Sections, 40 µm Thickness

Brain Case Test Data

For more information about related products and services, please contact us via email: BD@ebraincase.com
( *The content of this document is owned by Brain Case Biotech. Without explicit written consent from Brain Case Biotech, no individual or organization may reproduce, reprint, disseminate, or use the content of this document in any form )

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